The removal of 14C labeled endotoxin by activated charcoal.

摘要: Endotoxin shock due to Gram-negative enteric bacteria is of major medical concern with an estimated 100,000 fatalities in the United States per year. An effective therapy for endotoxin shock, particularly combination significant liver damage, has not been available date. Since activated charcoal known as a universal sorbent, use hemoperfusion apparatus remove interesting possibilities. Current assays are inadequate. The Limulus Amoebocyte Lysate (LAL) assay was found give nonreproducible results within our range requirements accuracy. We, therefore, grew Salmonella typhimurium 14C-labeled glucose obtain 14C labeled endotoxin. Radiolabeled used measure rate adsorption on charcoal. rates removal from normal saline, plasma, and whole blood will be presented graphical form design calculations. This work provides foundation encouraging vivo experimentation now underway at University Oklahoma Veteran's Administration Hospital City.