Differential inflammatory responses of bovine foot skin fibroblasts and keratinocytes to digital dermatitis treponemes.

摘要: Bovine digital dermatitis (BDD) is a serious infectious inflammatory lameness causing pain and suffering to many cattle worldwide which has severe economic implications. This study set out investigate relationships between the treponemes considered causal of BDD local response bovine host. Here we describe, for first time, isolation foot skin keratinocytes fibroblasts as separate cell lineages. These lines were then exposed treponeme whole-cell sonicates, gene expression selected host mediators investigated using quantitative reverse transcriptase PCR. Several genes, including those encoding RANTES/CCL5, MMP12, TNFα, TGFβ TIMP3 significantly upregulated in sonicates derived from phylotypes. For each above genes there similar fibroblast increases all three phylotypes tested, suggesting common virulence mechanisms. With keratinocytes, unable detect RANTES/CCL5 after incubation with constituents observe any significant changes tested. contrasting results suggest rather than may be an important shared target pathogenesis treponemes.