The effect of Hus1 on ionizing radiation sensitivity is associated with homologous recombination repair but is independent of nonhomologous end-joining
作者： X Wang , B Hu , R S Weiss , Y Wang
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摘要: Mammalian Hus1 plays an important role in maintaining genomic integrity. Cells lacking mouse are hypersensitive to DNA damage inducers including UV and camptothecin (CPT). By using clonogenic assay, we show here that deficient cells ionizing radiation (IR) compared with their Hus1-positive counterparts. However, these similar induction levels rejoining rates of double strand breaks (DSBs) following IR, indicating the effect on cell radiosensitivity is independent nonhomologous end-joining (NHEJ). combining I-SceI-induced-DNA DSBs system a siRNA approach, also knocking down decreases efficiency homologous recombination repair (HRR), which associated cellular sensitivity IR-induced killing. Together, results indicate affecting killing NHEJ but might be linked HRR.
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