Efficient Strategies for Analysis of Low Abundance Proteins in Plant Proteomics

摘要: Proteomic studies alongside with other post-genomic approaches are rapidly progressing in plant biology. This trend is reflected by the increased number of publications related to proteomics last several years. While proteomic span from global characterisation proteomes (the protein complements) whole plants and organs more detailed particular proteins, there an increasing demand for methods allowing sophisticated (and preferably quantitative) analysis a variety cells, compartments organelles, post-translational modifications, complexes their dynamics. The common problem high dynamic range proteome, many researchers looking ways improve recovery naturally low-abundant proteins. aim this chapter provide review approaches, rather than specific protocols, established isolation fractions proteins suitable mass spectrometry. We focus on sample preparation methodology general applications advances made plant-specific compartments. also describe advantages metabolic labeling techniques suited quantitative discuss tandem affinity purification perspectives single-cell plants.