Human gut microbiota in obesity and after gastric bypass

摘要: Recent evidence suggests that the microbial community in human intestine may play an important role pathogenesis of obesity. We examined 184,094 sequences 16S rRNA genes from PCR amplicons by using 454 pyrosequencing technology to compare structures 9 individuals, 3 each categories normal weight, morbidly obese, and post-gastric-bypass surgery. Phylogenetic analysis demonstrated although Bacteria intestinal were highly diverse, they fell mainly into 6 bacterial divisions had distinct differences study groups. Specifically, Firmicutes dominant normal-weight obese individuals but significantly decreased who a proportional increase Gammaproteobacteria. Numbers H2-producing Prevotellaceae enriched individuals. Unlike diverse Bacteria, Archaea comprised members order Methanobacteriales, which are H2-oxidizing methanogens. Using real-time PCR, we detected higher numbers H2-utilizing methanogenic than or The coexistence bacteria with relatively high gastrointestinal tract leads hypothesis interspecies H2 transfer between archaeal species is mechanism for increasing energy uptake large persons. population shift seen reflect double impact gut alteration caused surgical procedure consequent changes food ingestion digestion.