Determination of Phenolic Acids in Atriplex hortensis L. by Novel Solid-Phase Extraction and High-Performance Liquid Chromatography

摘要: ABSTRACTA novel offline solid-phase extraction high-performance liquid chromatography method is reported for the determination of phenolic acids in Atriplex hortensis L. Isolation analytes was performed by maceration followed with hydrophilic–lipophilic balance cartridges. The using C18 guard and analytical columns. mobile phase 0.08%, aqueous o-phosphoric acid 40:60 water/acetonitrile gradient eluation. were detected at 254 nm an ultraviolet detector. Calibration equations obtained least squares a linear dynamic range from 1.00 to 20.00 mg/L. limits detection quantification 0.25 0.45 0.77 1.36 mg/L, respectively. precision characterized as repeatability intermediate samples fortified low, medium, high concentrations. accuracy evaluated recovery. developed determined presence vanillic acid, syringic...