Functional complementation of a yeast knockout strain by Schistosoma mansoni Rho1 GTPase in the presence of caffeine, an agent that affects mutants defective in the protein kinase C signal transduction pathway.

摘要: In a previous study, the Schistosoma mansoni Rho1 protein was able to complement null mutant Saccharomyces cerevisiae cells at restrictive temperatures and under osmotic stress (low calcium concentration) better than human homologue (RhoA). It is known that stress, S. triggers two distinct pathways: activation of membrane 1,3-beta-glucan synthase enzymatic complex kinase C1 signal transduction pathway, promoting transcription response genes. present work SmRho1 its mutants smrho1E97P, smrho1L101T, L101T were used try clarify basis for differential complementation knockout yeast strain by Experiments functional in presence caffeine regulator sorbitol conducted. showed caffeine, since smrho1E97P delay growth when compared complemented with wild type SmRho1. However, similar phenotype, as they allowed all concentrations tested.