Naringin, a flavanone glycoside, promotes angiogenesis and inhibits endothelial apoptosis through modulation of inflammatory and growth factor expression in diabetic foot ulcer in rats.

摘要: Chronic, unhealed diabetic foot ulcer (DFU) is one of the most severe complications diabetes mellitus (DM). Naringin, a flavanone glycoside antioxidant, was reported to have antidiabetic and anti-apoptotic properties. In present study DM induced experimentally by streptozotocin (STZ, 55 mg/kg, i.p.). surgically introduced wounds on dorsal surface hind paw rats, healing potential naringin investigated. Rats were treated with (20, 40 80 p.o.), insulin (10 IU/kg, s.c.) tetrachlorodecaoxide (TCDO) (1 drop, twice day, topically) for 16 days. The wound area measured every second day 17 various biochemical parameters determined in serum, tissue, histopathological examination performed. Naringin (40 mg/kg) significantly (P<0.05) improved area, serum glucose level, glycated Hb insulin. treatment at mg/kg resulted significant up-regulation mRNA expression growth factor (IFG-1, TGF-β VEGF-c), Ang-1 collagen-1 whereas inflammatory mediators (TNF-α, IL-1β IL-6) down-regulated. Furthermore, attenuated STZ-induced apoptosis stimulated angiogenesis tissue. Further results suggest that via naringin-mediated inhibition hyperglycemia, oxidative stress, down-regulation mediator expression, leading DFU.