Novel phosphorylation sites of eukaryotic initiation factor-4F and evidence that phosphorylation stabilizes interactions of the p25 and p220 subunits.
作者： X. Bu , D.W. Haas , C.H. Hagedorn
DOI: 10.1016/S0021-9258(18)53491-8
关键词:
摘要: Only serine phosphorylation of eukaryotic initiation factor-4E (eIF-4E) has been previously reported in intact cells. We found that treatment HepG2 cells with okadaic acid resulted as much 20% factor (eIF)-4E occurring on threonine residues and tryptic phosphopeptide maps showed several unrecognized phosphopeptides. Analysis p220 from control acid-treated demonstrated under both conditions. However, a unique pattern phosphopeptides was observed. The most notable finding hyperphosphorylation eIF-4E increased binding but not to the m7GTP cap structure. suggest is more complicated than recognized recruits into protein complex associates mRNA caps. A better understanding these protein-protein protein-mRNA interactions may aid design anti-sense directed chemistries disrupt such for specific target (Baker, B.F., Miraglia, L., Hagedorn, C. H. (1992) J. Biol. Chem. 267, 11495-11499).
sci-hub.st 参考文章(23)
J A Grifo, S M Tahara, M A Morgan, A J Shatkin, W C Merrick, New initiation factor activity required for globin mRNA translation. Journal of Biological Chemistry. ,vol. 258, pp. 5804- 5810 ,(1983) , 10.1016/S0021-9258(20)81965-6
W Rychlik, M A Russ, R E Rhoads, Phosphorylation site of eukaryotic initiation factor 4E. Journal of Biological Chemistry. ,vol. 262, pp. 10434- 10437 ,(1987) , 10.1016/S0021-9258(18)60978-0
B J Lamphear, R Panniers, HEAT SHOCK IMPAIRS THE INTERACTION OF CAP-BINDING PROTEIN COMPLEX WITH 5' MRNA CAP Journal of Biological Chemistry. ,vol. 266, pp. 2789- 2794 ,(1991) , 10.1016/S0021-9258(18)49916-4
R Duncan, S C Milburn, J W Hershey, Regulated phosphorylation and low abundance of HeLa cell initiation factor eIF-4F suggest a role in translational control. Heat shock effects on eIF-4F. Journal of Biological Chemistry. ,vol. 262, pp. 380- 388 ,(1987) , 10.1016/S0021-9258(19)75938-9
S J Morley, J A Traugh, Phorbol esters stimulate phosphorylation of eukaryotic initiation factors 3, 4B, and 4F. Journal of Biological Chemistry. ,vol. 264, pp. 2401- 2404 ,(1989) , 10.1016/S0021-9258(19)81626-5
R W Donaldson, C H Hagedorn, S Cohen, Epidermal growth factor or okadaic acid stimulates phosphorylation of eukaryotic initiation factor 4F Journal of Biological Chemistry. ,vol. 266, pp. 3162- 3166 ,(1991) , 10.1016/S0021-9258(18)49968-1
W Rychlik, J S Rush, R E Rhoads, C J Waechter, Increased rate of phosphorylation-dephosphorylation of the translational initiation factor eIF-4E correlates with the induction of protein and glycoprotein biosynthesis in activated B lymphocytes. Journal of Biological Chemistry. ,vol. 265, pp. 19467- 19471 ,(1990) , 10.1016/S0021-9258(17)45396-8
B J Lamphear, R Panniers, Cap binding protein complex that restores protein synthesis in heat-shocked Ehrlich cell lysates contains highly phosphorylated eIF-4E. Journal of Biological Chemistry. ,vol. 265, pp. 5333- 5336 ,(1990) , 10.1016/S0021-9258(19)39358-5
S J Morley, T E Dever, D Etchison, J A Traugh, Phosphorylation of eIF-4F by protein kinase C or multipotential S6 kinase stimulates protein synthesis at initiation. Journal of Biological Chemistry. ,vol. 266, pp. 4669- 4672 ,(1991) , 10.1016/S0021-9258(19)67700-8
B.F. Baker, L Miraglia, C.H. Hagedorn, Modulation of eucaryotic initiation factor-4E binding to 5'-capped oligoribonucleotides by modified anti-sense oligonucleotides. Journal of Biological Chemistry. ,vol. 267, pp. 11495- 11499 ,(1992) , 10.1016/S0021-9258(19)49937-7