Infection by Streptococcus pyogenes Induces the Receptor Activator of NF-κB Ligand Expression in Mouse Osteoblastic Cells

摘要: Group A Streptococcus pyogenes is known to induce nongonococcal septic arthritis in addition pharyngitis, scarlet fever, and poststreptococcal sequelae. However, little about the interaction between S. bone cells. We report here that strain JRS4 (M6) attached invaded mouse primary osteoblasts. Reverse transcription-PCR demonstrated infection of osteoblasts stimulated expression mRNA for receptor activator NF-kappaB ligand (RANKL). Western blot analysis followed by precipitation with showed there was an increase RANKL protein infected Production interleukin-6 also stimulated, but no production interleukin-1beta or tumor necrosis factor alpha observed. Stimulation not observed heat-inactivated pyogenes, suggesting active essential this phenomenon. performed antibodies specific phosphorylated signal transduction proteins induces phosphorylation p38 mitogen-activated kinase. inhibitor kinase, SB203580, inhibited These results suggest stimulates may trigger destruction tissue.