Determination of artemether and its metabolite, dihydroartemisinin, in plasma by high-performance liquid chromatography and electrochemical detection in the reductive mode

摘要: Abstract An analytical method for the determination of artemether (A) and its metabolite dihydroartemisinin (DHA) in human plasma has been developed validated. The is based on high-performance liquid chromatography (HPLC) electrochemical detection reductive mode. A, DHA artemisinin, internal standard (I.S.), were extracted from (1 ml) with 1-chlorobutane—isooctane (55:45, v/v). solvent was transferred, evaporated to dryness under nitrogen residue dissolved 600 μl water-ethyl alcohol (50:50, Chromatography performed a Nova-Pak CN, 4 μm column (150 mm×3.9 mm I.D.) at 35°C. mobile phase consisted pH 5 acetate—acetonitrile (85:15, v/v) flow-rate 1 ml/min. analytes detected by mode potential −1.0 V Intra-day accuracy precision assessed relative recoveries (found concentration % nominal value) spiked samples analysed same day (concentration range 10.9 202 ng/ml A 11.2 206 plasma). mean over entire 96 100% C .V. 6 13%, 92% (α-tautomer) 16%. For recovery 96% limit quantitation (LOQ) CV 13%. DHA, LOQ