The Sequence of SCMK-B2B, a High-Sulfur Protein from Wool Keratin
作者： Thomas C. Elleman , Theo A. Dopheide
DOI: 10.1016/S0021-9258(19)45120-X
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摘要: Abstract The complete amino acid sequence of the protein subfraction B S-carboxymethylated high-sulfur from reduced and wool has been determined. contains 156 residues; NH2-terminal alanine residue is acetylated COOH-terminal S-carboxymethylcysteine. no lysine, histidine, nor methionine, only 6 arginine residues. Only six carboxyl side chains are present in addition to 34 residues derived Tryptic digestion this gave four peptides containing 2, 7, 59, 88 structures these were determined by enzymic cleavage with thermolysin, pepsin, chymotrypsin, use Edman degradation dansylation. Protein SCMK-B2B displays a high degree internal homology.
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