The Scr Circuit in Vibrio parahaemolyticus Modulates Swarming and Sticking

摘要: The role of c-di-GMP in determining the switch between motile and sessile lifestyles appears to be an emerging theme many bacteria; some bacteria, one important consequence is impact on virulence. Scr (swarming capsular polysaccharide gene regulation) genes influence lifestyle adaptation swarming sticking by modulating cellular nucleotide pool. Fractionation experiments localize ScrA cytoplasm, ScrB periplasm, ScrC with cell membrane. Curiously, overproduction without coproduction does not simply result loss increased laf expression but rather recapitulates ScrABCΔEAL phenotype. In contrast, both GGDEF EAL domains possess catalytic activity: itself (in absence ScrB), can synthesize Vibrio parahaemolyticus as well Escherichia coli), whereas context functional ScrB, capable degrading this secondary messenger. comparison, activity seems modulated ScrB. enzymes controlling level include proteins phosphohydrolase-associated HD-GYP domain. possessing a multiplicity types appropriate for life under different circumstances, swimmer, swarmer, sticky types, ubiquitous marine bacterium human pathogen V. must make certain critical determinations. plays key influencing decision-making process.