Definition of a specific interaction between the early T lymphocyte activation 1 (Eta-1) protein and murine macrophages in vitro and its effect upon macrophages in vivo.

摘要: The Eta-1 gene specifies a secreted product of activated T cells and is associated with genetic resistance to infection by an obligate intracellular bacterium. Previous studies have suggested that eta-1 might affect the ability macrophages migrate site bacterial and/or inhibit growth. We therefore examined interaction in vitro vivo. find express approximately 10(4) receptors/cell each receptor has Kd 5 x 10(-10) M. subsequence containing RGD motif required for binding because synthetic peptide domain inhibited protein attachment macrophages. also found subcutaneous inoculation mice resulted cellular infiltrate comprised primarily propose between its on results change macrophage physiology resulting accumulation these at extravascular sites.