Antiproliferative Activity of a Pregnancy Recognition Hormone, Ovine Trophoblast Protein-1

摘要: Ovine trophoblast protein-1 (oTP-1) is the alpha-interferon (IFN alpha) variant, secreted by conceptuses and referred to as type I interferon, that responsible for maternal recognition of pregnancy in sheep. We have previously shown oTP-1 potent an antiviral agent any known IFN. IFNs also possess anticellular activity are, fact, used cancer therapy been found be effective treatment such myelogenous hairy cell leukemias. A significant problem with currently undesirable side effect toxicity at high concentrations. In this study, we examined oTP-1. It inhibited proliferation but did not exhibit concentrations, unlike IFN alpha S. assay using colony formation both human amnionic line, WISH, bovine epithelial MDBK, size number. was s on cells, respectively; thus, it displays cross-species activity. Its dose dependent, inhibition could observed concentrations low 1 unit/ml. Concentrations 50,000 units/ml stopped proliferation, while viability impaired. Cell cycle analysis revealed increased proportion cells S phase a corresponding decreased G2/M after 48 h treatment. Therefore, appears inhibit progress through phase. antiproliferative effects can early 12 initiation culture are maintained 6 days. Thus, exhibits without across species may therapeutic potential antitumor toxic generally associated IFNs.