Low-phosphate-selected Auxenochlorella protothecoides redirects phosphate to essential pathways while producing more biomass.

摘要: Despite the capacity to accumulate ~70% w/w of lipids, commercially produced unicellular green alga A. protothecoides may become compromised due high cost phosphate fertilizers. To address this limitation was selected for adaptation conditions 100× and 5× lower peptone, respectively, compared ‘wild-type media’. The showed initial signs by 45–50 days, steady state growth at ~100 days. low (P)-adapted strain up ~30% greater biomass, while total lipids (~10% w/w) remained about same, wild-type strain. Metabolomic analyses indicated that P-adapted 3.3-fold more saturated palmitic acid (16:0) 2.2-fold less linolenic (18:3), strain, resulting in an ~11% increase caloric value, from 19.5kJ/g 21.6kJ/g amounts composition certain fatty acids, wild type Biochemical changes adapted were assessed comparative RNA-Seq analysis, which yielded 27,279 transcripts. Among them, 2,667 15 genes significantly down- up-regulated, >999-fold >3-fold (adjusted p-value <0.1), respectively. expression encoding proteins involved cellular processes such as division, growth, membrane biosynthesis, a trend toward down-regulation. At genomic level, synonymous SNPs Indels observed primarily coding regions, with 40S ribosomal subunit gene harboring substantial SNPs. Overall, out-performed prioritizing use its limited supply essential biological processes. is expected be economical grow over based on overall productivity content, importantly, also requiring 100-fold phosphate.