Left-Handed Z-DNA and Genetic Recombination

摘要: Publisher Summary Genetic recombination is the process by which DNA translocated from one region of chromosome to another. Recombinational events are classified into two basic groups: homologous (general) and site-specific recombination. This chapter discusses mechanisms Homologous occurs between regions extensive homology essentially anywhere on chromosome. The integration bacteriophage lambda Escherichia coli best illustrates describes left­­-handed Z-DNA. Z-DNA a higher energy form than B-DNA; it requires presence stabilizing agent such as high salt, divalent metal ions, base modifications (for example, halogenation methylation), or negative supercoiling. exists in oligonucleotides, polymers, restriction fragments, recombinant plasmids. Tracts left-handed can be contiguous with right-handed B-DNA, thus necessitating B-Z junctions. Recently, was shown exist elicit biological responses vivo.