Myofibroblasts are important contributors to human hepatocellular carcinoma: Evidence for tumor promotion by proteome profiling

摘要: Liver cancer typically occurs with a background of chronic fibrosis, characterized by the accumulation myofibroblast-like cells. We performed 2D-PAGE-based comparative analyses aim to identify proteins expressed in human hepatocellular carcinoma (HCC) tissue but not neighboring healthy liver tissue, and make out which cell types are responsible for expression most characteristic HCC. LC-MS/MS analysis striking spots identified that were mainly related To gain more insights into role these cells their contribution HCC, we isolated as well hepatocytes, both derived from HCC tissues, subjected them proteome profiling based on shotgun experiments. Comparative analysis, also referring profiles other previously investigated us, pointed again marked Intriguingly, secretome several growth factors may act tumor promoters have been described potential biomarkers including dickkopf-1, connective factor, CXCL1. Other biomarker candidates presently myofibroblasts, lipocalin-1 pappalysin-1, be selected future clinical validation. The identification important source tumor-promoters open new avenues therapeutic intervention targeting stroma addition