Mechanical loading stimulates cell hypertrophy and specific gene expression in cultured rat cardiac myocytes. Possible role of protein kinase C activation.

摘要: To examine the molecular mechanisms by which mechanical stimuli induce cardiac hypertrophy and specific gene expression, we cultured rat neonatal cardiocytes in deformable dishes imposed an vitro load stretching adherent cells. Myocyte increased total cell RNA content mRNA levels of c-fos skeletal alpha-actin. Nuclear run-off transcription assay revealed that this increase level at least partially reflects changes transcriptional status. The transfected chloramphenicol acetyltransferase linked to upstream sequences fos indicated containing a serum response element were required for efficient cAMP/calcium might not be involved myocyte stretching. accumulation was suppressed protein kinase C inhibitors inhibited markedly down-regulation C. Moreover, inositol phosphate levels, activation phorbol esters stimulated expression alpha-actin genes. These findings suggest (myocyte stretching) directly possibly via activation.