Synthesis, characterization and in vitro cytotoxicity assessment of hydroxyapatite from different bioresources for tissue engineering application
作者： SUDIP MONDAL , RAJASHREE BARDHAN , BISWANATH MONDAL , APURBA DEY , SUDIT S MUKHOPADHYAY
DOI: 10.1007/S12034-012-0346-Y
关键词:
摘要: In the present study, hydroxyapatite (HAp) is synthesized from different biosources like eggshell, fish scale and bovine bone in a cost effective ecofriendly way. HAp materials were eggshell by wet precipitation method whereas thermal decomposition was applied case of bone. The phase purity crystallinity calcined powder determined XRD FTIR analyses. thermogravimetric analysis carried out to show stability powder. Average grain sizes sintered samples submicron range. morphology powders observed under scanning electron microscopy (SEM). dried ball milled for several hours surfactants Triton-X small fillers (2 / 4 mm long rod-shaped) made vitro testing. order verify biocompatibility powders, cytotoxicity evaluation RAW macrophage cell line media an incubation period 72 h. attachment studies on compacts excellent affinity between cells compact surface. These results proved high obtained tissue engineering applications.
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