Analysis of radiation therapy in a model of triple-negative breast cancer brain metastasis.

摘要: Most cancer patients with brain metastases are treated radiation therapy, yet this modality has not been meaningfully incorporated into preclinical experimental metastasis models. We applied two forms of whole therapy (WBRT) to the brain-tropic 231-BR model triple-negative breast cancer. When compared sham controls, WBRT as 3 Gy × 10 fractions (3 10) reduced number micrometastases and large by 87.7 54.5 %, respectively (both p < 0.01); whereas a single dose 15 1 (15 1) was less effective, reducing 58.4 % (p 0.01) 47.1 = 0.41), respectively. Neuroinflammation in adjacent parenchyma due solely reaction from metastases, radiotherapy, while adult neurogenesis brains adversely affected following both regimens. The nature resistance investigated ex vivo culture tumor cells that survived initial ("Surviving" cultures). Surviving cultures surprisingly demonstrated increased radiosensitivity vivo. In contrast, re-injection re-treatment regimen significantly developed vivo, suggesting role for microenvironment. Micrometastases derived surviving trend toward radioresistance upon repeat treatment 0.09). data confirm potency fractionated identify microenvironment potential determinant efficacy. also nominate new translational radiotherapy.