作者: Alison R. Sherwood , Akira Kurihara , Kimberly Y. Conklin
DOI: 10.1111/J.1440-1835.2010.00591.X
关键词:
摘要: SUMMARY Sixty-one Hawaiian algal specimens corresponding to members of the tribe Amansieae (Amansia and Osmundaria) were compared through DNA sequence analysis. Short barcode-like sequences mitochondrial cytochrome c oxidase subunit I (COI) universal plastid amplicon (UPA) markers obtained for as many possible, a subset was also used amplification sequencing nuclear small-subunit rRNA (SSU) gene phylogenetic inference in broader taxonomic context. Statistical parsimony analysis COI UPA A. glomerata produced relationships among samples that largely congruent with each other, although marker more conserved. The yielded three lineages, nucleotide divergences these lineages intermediate those typically reported intraspecific interspecific comparisons, suggesting they represent either incipient species or complex closely related species. demonstrated little no divergence Osmundaria obtusiloba taxon referred Amansia fimbrifolia. In contrast, identified daemelii identical lineage 3 glomerata, it is recommended this longer be included lists flora. Phylogenetic reconstruction based on SSU unresolved, indicating may limited utility purpose group algae, but small amount variation found glomerata.