Aptamers specific for biomolecules and methods of making

摘要: A method for identifying oligomer sequences, optionally comprising modified base, which specifically bind target molecules such as serum proteins, kinins, eicosanoids and extracellular proteins is described. The used to generate aptamers that Factor X, PDGF, FGF, ICAM, VCAM, E-selectin, thrombin, bradykinin, PGF2 cell surface molecules. technique involves complexation of the molecule with a mixture oligonucleotides containing random sequences serve primer PCR under conditions wherein complex formed binding but not other members oligonucleotide mixture. then separated from uncomplexed complexed are recovered using polymerase chain reaction. may be sequenced, successive rounds selection complexation, separation, amplification recovery can employed. therapeutic diagnostic purposes generating secondary aptamers.