Systematic analysis of the Myxococcus xanthus developmental gene regulatory network supports posttranslational regulation of FruA by C-signaling.

摘要: Upon starvation Myxococcus xanthus undergoes multicellular development. Rod-shaped cells move into mounds in which some differentiate spores. Cells begin committing to sporulation at 24-30 h poststarvation, but the mechanisms governing commitment are unknown. FruA and MrpC transcription factors that necessary for commitment. They bind cooperatively promoter regions activate developmental gene transcription, including of dev operon. Leading up during period, mRNA increased wild type, not a mutant defective C-signaling, short-range signaling interaction between is also The C-signaling exhibited ~20-fold less than type 30 despite similar level only 2-fold FruA. Boosting twofold had little effect on level, was stable mutant. Neither did high cooperativity binding upstream explain data. Rather, our systematic experimental computational analyses support model activates least ninefold posttranslationally order commit cell spore formation.