Requirement for PCNA in DNA mismatch repair at a step preceding DNA resynthesis
作者： Asad Umar , Andrew B Buermeyer , Jeffrey A Simon , David C Thomas , Alan B Clark
DOI: 10.1016/S0092-8674(00)81323-9
关键词:
摘要: Abstract id system was used to screen yeast and human expression libraries for proteins that interact with mismatch repair proteins. PCNA recovered from both shown in the case of MLH1 MSH2. A strain containing a mutation gene had strongly elevated rate dinucleotide repeat, not further also . Mismatch activity examined cell extracts using an assay does require DNA synthesis. Activity inhibited by p21 WAF1 or peptide, which bind PCNA, restored reactions addition PCNA. The data suggest requirement at step preceding resynthesis. ability MSH2 may reflect linkage between replication be relevant roles other transactions.
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