Lipase in lipid bodies of cotyledons of rape and mustard seedlings.

摘要: Lipolytic activity was absent in the crude cotyledon extract of ungerminated rapeseed (Brassica napm L. var. Dwarf Essex), and increased to a peak at day 4 seedling growth, concomitant with decrease total lipids. About 50% lipase recovered lipid bodies isolated from by flotation centrifugation. Isolated underwent autolysis internal triacylglycerols resulting release fatty acids. After had been extracted diethyl ether, remaining membrane fraction. The maximal pH 6.5 on trierucin, trilinolein, or endogenous triacylglycerols, 8.0 N-methylindoxylmyristate. most active trierucin hydrolyzed related di- monoacylglycerols lower rates. There little enhancement presence NaCl, CaCl2, detergents, detergents general reduced activity. hydrolysis linear until about converted erucic acid, there accumulation dierucin monoerucin. Lipase germinated variety Tower, which contains no acids storage also highest activities trilinolein. A comparative study mustard seed juncea) revealed that possessed characteristics very similar those lipase.