Anti-hepatoma cells function of luteolin through inducing apoptosis and cell cycle arrest
作者： Shixiong Ding , Airong Hu , Yaoren Hu , Jianbo Ma , Pengjian Weng
DOI: 10.1007/S13277-013-1396-5
关键词:
摘要: The aim of this study is to explore the apoptotic induction and cell cycle arrest function luteolin on liver cancer cells related mechanism. line SMMC-7721, BEL-7402, normal HL-7702 were treated with different concentrations luteolin. Cell proliferation ability was tested. Morphological changes observed under inverted fluorescence microscope after Hoechst33342 staining. We investigated effect cycling apoptosis flow cytometry. mitochondrial membrane potential analyzed JC-1 Caspases-3 Bcl-2 family proteins expression by real-time PCR. SMMC-7721 BEL-7402 inhibited luteolin, inhibition dose–time-dependent. Luteolin could at G1/S stage, reduce potential, induce higher rate typical morphological carcinoma cells. Q-RT-PCR results also showed that increased Bax caspase-3 significantly upregulated in a dose-dependent manner However, almost not affected treatment. can inhibit time- manner. And mechanism maybe through arresting phase G1/S, enhancing level, reducing anti-apoptotic protein resulting activating enzyme decrease finally leading apoptosis.
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