Histochemical localization of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine oxidation in the mouse brain

摘要: The sites in the mouse brain where 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine can be oxidized to toxic metabolite 1-methyl-4-phenylpyridine were determined using a histochemical technique. method involved demonstration of monoamine oxidase activity as substrate by means sensitive coupled peroxidase distribution neurons displaying ability oxidize via catalysed reaction was compared that various amine systems identified with immunohistochemistry. Dopamine neurons, and particular nigrostriatal dopamine cells, did not display capacity 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine. Noradrenergic showed intense when used substrate, this blocked oxidase-A inhibitor clorgyline. Serotonin histamine also able these deprenyl, an oxidase-B. Pretreatment inhibitors oxidase-B has been previously shown prevent neurotoxic action on dopaminergic neurons. Therefore, since serotonin are oxidase-B, may production metabolites l-methyl-4-phenyl-1,2,3,6-tetrahydropyridine vivo.