A self-calibrating electrochemical aptasensing platform: Correcting external interference errors for the reliable and stable detection of avian influenza viruses

摘要: Abstract Conventional electrochemical biosensing systems rely on a single output signal, which limits their certain practical application, specifically from the viewpoint of external interference factors causing signal errors. This study reports self-calibrating dual-electrode based aptasensor for reliable and independent detection avian influenza viruses (AIVs), are primary cause highly contagious respiratory diseases, under factors. Both electrodes were fabricated using tungsten rods surface-modified with 3D nanostructured porous silica film (3DNRE). Subsequently, methylene blue (MB) was loaded as redox-active material into pores capped corresponding aptamer. One electrode an anti-AIV nucleoprotein (NP) aptamer (AptAIV-MB@3DNRE) allowing target-specific binding, resulting in changes upon diffusional release redox molecules. The other control (Aptcon-MB@3DNRE), serving reference to correct false responses generated by nonspecific detachment MB environmental pH ion strength presence nontarget molecules cell lysis debris. In platform, Aptcon-MB@3DNRE provides corrected baseline fluctuating original signals AptAIV-MB@3DNRE. Consequently, this platform exhibits excellent output-signal stability (relative standard deviation, RSD: 5.86%) compared conventional single-electrode (RSD: 30.13%) at equivalent concentrations AIV NP samples different reaction buffer conditions. Moreover, no further purification washing steps required, indicating that strategy may represent universal aptamer-based various biomolecules.