An in vitro traumatic injury model to examine the response of neurons to a hydrodynamically-induced deformation.

摘要: A novelin vitro system was developed to examine the effects of traumatic mechanical loading on individual cells. The cell shearing injury device (CSID) is a parallel disk viscometer that applies fluid shear stress with variable onset rate. CSID used in conjunction microscopy and biochemical techniques obtain quantitative expression deformation functional response neurons injury. Analytical numerical approximations at bottom were compared determine contribution secondary flows. significant portion directed ther-direction during start-up, therefore full Navier-Stokes equation necessary accurately describe transient stress. When applied high rate (800 dyne cm−2 sec−1) cultured neurons, range membrane strains (0.01 0.53) obtained, suggesting inhomogeneity cellular response. Functionally, cytosolic calcium extracellular lactate dehydrogenase levels increased strain (>1 loading, quasistatic (<1 loading. In addition, subpopulation culture subjected rapid subsequently died. These rates are relevant those shown occur injury, as such, an appropriate model for studying biomechanics pathophysiology neuronal