VARIABILITY IN THE BINDING OF ANTI-MAG AND ANTI-SGPG ANTIBODIES TO TARGET ANTIGENS IN DEMYELINATING NEUROPATHY AND IGM PARAPROTEINEMIA

摘要: Densitometry of immunostained Western blots or thin layer chromatograms and enzyme-linked immunosorbent assays (ELISAs) were used to compare the relative strengths IgM binding myelin-associated glycoprotein (MAG), P0 glycoprotein, peripheral myelin protein-22 (PMP-22), sulfate-3-glucuronyl paragloboside (SGPG), other potential target antigens in a series eleven patients with sensory sensorimotor demyelinating neuropathy paraproteinemia. The from all exhibited reactivity both MAG SGPG, there was statistically significant correlation between overlay ELISAs for measuring strength SGPG. However, data revealed variations which antibodies bound heterogeneity their fine specificities. First, poor respectively. Second, SGPG few only detected by one two assay systems. Third, about one-third patients' absolutely required sulfate on reactivity, whereas others retained some after removal sulfate. Fourth, unusually strong proteins compact myelin, PMP22. These differences antibody compared clinical presentations responses intravenous immunoglobulin (IVIg) therapy trial they participated. For most part, these did not correlate presentation, relatively homogeneous this patients. an inverse relationship noted degree ELISA response IVIg. Two who responded had mild elevations nerve glycoconjugates unusual immunochemical characteristics comparison results demonstrate anti-MAG anti-SGPG different bind neural may affect pathogenic mechanisms therapy.