Solution structure of the fifth repeat of factor H: a second example of the complement control protein module.

摘要: Modules which share the same consensus sequence are assumed to have common structural features, at secondary and tertiary level. In order test extent of such similarities, it is necessary examine structures several examples from each module family. Recently, first three-dimensional structure a complement control protein (CCP) (the 16th repeat human factor H, H16) was determined using combination two-dimensional NMR simulated annealing (Norman, D. G., Barlow, P. N., Baron, M., Day, A. J., Sim, R. B., & Campbell, I. (1991) J. Mol. Biol. 219, 717-7251. Using techniques, second CCP 5th H5) has now been determined. The primary H5 contains 17 residues identical in equivalent position those H16. Thirteen these part sequence. similarities between that H16 extensive. This implies dictates particular structure. H5, compact hydrophobic core wrapped P-strand sheet, bears much overall resemblance However, there deletion strand an insertion loop, resulting slightly shorter length. associated with rearrangement within core. side chain highly conserved Tyr29, occupies central H16, lies on periphery H5.