Exocellular chitinase from a Streptomyces sp.

摘要: SUMMARY: A number of cultures chitin-decomposing aerobic bacteria were isolated by the enrichment technique. Chitin decomposition was followed measurement loss alkali-resistant insoluble substrate. Agitated submerged secured a much more rapid breakdown chitin than static cultures. The exocellular chitinase produced species Streptomyces in agitated culture studied. effects pH value and substrate concentration on enzyme activity examined results used to establish assay terms reducing-sugar production. an inducible one (adaptive). concentrated ultrafiltration lyophilization. water-soluble reducing materials from identified as N-acetylglucosamine its corresponding disaccharide, N.N-diacetylchitobiose.