Searching for clones with open reading frames.

摘要: Publisher Summary This chapter has devised a simple strategy to quickly locate and express open reading frames. Using this approach, it is possible identify protein coding regions without any extensive information about the products. One of distinguishing features deoxyribo nucleic acid (DNA) in protein-coding presence at least one frame (ORF). DNA sequences outside regions, such as intergenic introns, rarely have long Typically, order characterize region DNA, its sequence, ribo (RNA), or products are used construct molecular map. The approach described can be whose either unknown undetectable, starting with cloned viral DNA. same portion poorly characterized well-characterized complementary (cDNA). Antibodies made against polypeptide determinants encoded by