Detection and cloning of unique integration sites of retrotransposon, intracisternal A‐particle element in the genome of acute myeloid leukemia cells in mice
作者： H Ishihara , I Tanaka
DOI: 10.1016/S0014-5793(97)01383-5
关键词:
摘要: We previously found retrotransposition of the intracisternal A-particle (IAP) element in genome acute myeloid leukemia (AML) cells induced by X-irradiation C3H/He mice (FEBS 16333). To analyze occurrence IAP-mediated AML cells, we compared integration sites IAP polymerase chain reaction (PCR) genomes five strains derived from different C3H mice. Unique PCR products were all above independent whereas no such detected normal cells. Results cloning, sequencing and Southern analyses showed that novel genome. The data suggest occurs frequently radiation-induced
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