Contribution by different fuels and metabolic pathways to the total ATP turnover of proliferating MCF-7 breast cancer cells.

摘要: For the past 70 years dominant perception of cancer metabolism has been that it is fuelled mainly by glucose (via aerobic glycolysis) and glutamine. Consequently, investigations into diagnosis, treatment basic cells have directed this perception. However, data on are equivocal, in study we sought to clarify issue. Using an innovative system measured total ATP turnover MCF-7 breast cell line, contributions oxidative glycolytic production component glucose, lactate, glutamine, palmitate oleate. The over approx. 5 days was 26.8 micromol ATP.10(7) cells(-1).h(-1). 80% 20% glycolytic. Contributions were 10% 14% 7% palmitate, 4% oleate 65% from unidentified sources. contribution (glycolysis oxidation) 28.8%, glutamine contributed 10.7% combined 40%. Glucose significant fuels, but they account for less than half turnover. glycolysis not different a variety other non-transformed types.