Development of a Modular Ratiometric Fluorescent Probe for the Detection of Extracellular Superoxide
作者： Diana Andina , Davide Brambilla , Noah Munzinger , Jasmine Frei , Cristina Zivko
关键词:
摘要: Extracellular detection of endogeneous analytes (e.g., superoxide) can provide important insights into mechanisms homeostasis and diseases, such as tumorigenesis. A ratiometric probe with a fluorescent reference an analyte-specific switch-on dye was developed. Detection ROS in the extracellular milieu ensured by connecting two fluorophores modular peptide-nucleic-acid-based linker. The ROS-sensing ability assessed validated cell-free assays cell culture.
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