Composition and synthesis in vivo of water-insoluble proteins in the rat ocular lens: Normal and cataractous

摘要: Abstract This study describes the composition and synthesis in vivo of water-insoluble proteins normal cataractous rat ocular lens. Cataracts lens nucleus were induced by treatment young with U18666A, 3β-(2-diethylaminoethoxy)androst-5-en-17-one. HCl. Opaque contained more insoluble incorporated intraocularly injected [ 3 H]leucine into these than clear Insoluble from both opaque consisted classes three mol. wt. ranges; > 68 000, 35 000–60 000 16 000–22 000. Treatment mercaptoethanol produced changes electrophoretic profiles lens; however, higher class only was reduced to smaller components. The lowest accounted for over 50% total protein types roughly proportionate their relative concentrations, although there a tendency incorporate label peak 1 2 proteins. In general, greater concentration this cataract appeared due proportionately increased formation same present contrast human senile cataracts, did not preferentially accumulate disulfidelinked very high protein. However, aged (i.e. pre-existing) U18666A different those newly synthesized, since under conditions affecting synthesized.