Amino group reactivity in DNA-aminoacridine complexes

摘要: The rates of diazotization representatives two classes dyes within the acridine and triphenylmethane families have been studied under three sets conditions: (1) free in solution; (2) bound to DNA; (3) dissimilar anionic polymers. are distinguished according possibility or impossibility fitting all amino groups each molecule area bounded by external van der Waals contour projection a base pair onto plane dye. For neither class dye is reaction rate relative simple solution inhibited more than 50%, usually much less, combination with synthetic However, sharply effect DNA binding, that can fall inside diagram found be strongly inhibited, while those lying outside relatively unaffected. It concluded DNA, unlike other polymers, covers both faces Since no formaldehyde-reactive nucleotides liberated dye, hydrogen-bonding between pairs not significantly disturbed. These structural requirements agreement intercalation model has previously proposed.