LSC Abstract – Is it possible to predict the outcome of the whole lung lavage (WLL) in pulmonary alveolar proteinosis (PAP)?

摘要: PAP is a rare disorder characterized by the accumulation of lipoproteinaceous material within alveolar spaces. WLL current standard treatment for patients and although it effective in majority cases, disease persistence not unusual. To identify blood biomarkers able to predict outcome WLL: total resolution/persistent improvement (positive outcome) or transient resolution/progressive deterioration (negative outcome). We collected RNA from peripheral mononuclear cells 8 who underwent WLL, at baseline 24 months lavage. After 24-month follow up, they were dichotomized positive vs negative microarray analysis was performed. Co-expression eigengenes module search Gene Ontology, Reactome, KEGG, Disease Ontology enrichment performed differentially expressed genes (DEGs). Our preliminary data show 86 significantly DEGs, between with outcome; whereas seems induce difference gene expression, same subject, least after two years. The co-expression networks analysis, selected comprehending 764 genes, which showed significance mean p value=0.04 strong relationship pairwise connections significance. common predominance GO biological process Reactome pathways. This study allows key pathways whose different expression could discriminate outcome. validation our results will lead us select useful up clinical response WLL.