Outer membrane defect and stronger biofilm formation caused by inactivation of a gene encoding for heptosyltransferase I in Cronobacter sakazakii ATCC BAA-894.
作者： L. Wang , X. Hu , G. Tao , X. Wang
DOI: 10.1111/J.1365-2672.2012.05263.X
关键词:
摘要: Aims: To investigate the role of lipopolysaccharide (LPS) structure in stability outer membrane and ability biofilm formation Cronobacter sakazakii. Methods Results: A C. sakazakii mutant strain LWW02 was constructed by inactivating gene ESA_04107 encoding for heptosyltransferase I. LPS were purified from LWW02, changes their confirmed thin-layer chromatography electrospray ionization mass spectrometry. Comparing with wild-type BAA-894, slower growth, higher permeability, surface hydrophobicity, stronger autoaggregation observed LWW02. Conclusions: The encodes I ATCC BAA-894. cleavage could cause its defects increase to form biofilms. Significance Impact Study: study is important understanding pathogenic mechanism efficient control C. sakazakii.
sci-hub.se 参考文章(64)
ANGELIKA LEHNER, KATHRIN RIEDEL, LEO EBERL, PIETER BREEUWER, BENJAMIN DIEP, ROGER STEPHAN, Biofilm formation, extracellular polysaccharide production, and cell-to-cell signaling in various Enterobacter sakazakii strains: aspects promoting environmental persistence. Journal of Food Protection. ,vol. 68, pp. 2287- 2294 ,(2005) , 10.4315/0362-028X-68.11.2287
L Chen, W G Coleman, Cloning and characterization of the Escherichia coli K-12 rfa-2 (rfaC) gene, a gene required for lipopolysaccharide inner core synthesis. Journal of Bacteriology. ,vol. 175, pp. 2534- 2540 ,(1993) , 10.1128/JB.175.9.2534-2540.1993
N. R. Mullane, J. Murray, D. Drudy, N. Prentice, P. Whyte, P. G. Wall, A. Parton, S. Fanning, Detection of Enterobacter sakazakii in Dried Infant Milk Formula by Cationic-Magnetic-Bead Capture Applied and Environmental Microbiology. ,vol. 72, pp. 6325- 6330 ,(2006) , 10.1128/AEM.03056-05
Timothy C. Meredith, Parag Aggarwal, Uwe Mamat, Buko Lindner, Ronald W. Woodard, Redefining the requisite lipopolysaccharide structure in Escherichia coli. ACS Chemical Biology. ,vol. 1, pp. 33- 42 ,(2006) , 10.1021/CB0500015
Chan Zhang, Yanyan Li, Guanjun Tao, Ye Li, Xiaoyuan Wang, Characterization of lipid A Cronobacter sakazakii. European Journal of Mass Spectrometry. ,vol. 16, pp. 531- 538 ,(2010) , 10.1255/EJMS.1074
Carol Iversen, Angelika Lehner, Niall Mullane, Eva Bidlas, Ilse Cleenwerck, John Marugg, Séamus Fanning, Roger Stephan, Han Joosten, The taxonomy of Enterobacter sakazakii: proposal of a new genus Cronobacter gen. nov. and descriptions of Cronobacter sakazakii comb. nov. Cronobacter sakazakii subsp. sakazakii, comb. nov., Cronobacter sakazakii subsp. malonaticus subsp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov. and Cronobacter genomospecies 1 BMC Evolutionary Biology. ,vol. 7, pp. 64- 64 ,(2007) , 10.1186/1471-2148-7-64
N. Mullane, P. O'Gaora, J. E. Nally, C. Iversen, P. Whyte, P. G. Wall, S. Fanning, Molecular analysis of the Enterobacter sakazakii O-antigen gene locus Applied and Environmental Microbiology. ,vol. 74, pp. 3783- 3794 ,(2008) , 10.1128/AEM.02302-07
T. Osaili, S. Forsythe, Desiccation resistance and persistence of Cronobacter species in infant formula. International Journal of Food Microbiology. ,vol. 136, pp. 214- 220 ,(2009) , 10.1016/J.IJFOODMICRO.2009.08.006
S.R Sudhamani, R.N Tharanathan, M.S Prasad, Isolation and characterization of an extracellular polysaccharide from Pseudomonas caryophylli CFR 1705 Carbohydrate Polymers. ,vol. 56, pp. 423- 427 ,(2004) , 10.1016/J.CARBPOL.2004.03.008
Rob Van Houdt, Chris W. Michiels, Role of bacterial cell surface structures in Escherichia coli biofilm formation Research in Microbiology. ,vol. 156, pp. 626- 633 ,(2005) , 10.1016/J.RESMIC.2005.02.005