Morphologic and genomic characterization of a broad host range Salmonella enterica serovar Pullorum lytic phage vB_SPuM_SP116.

摘要: For effective use of phages as antimicrobial agents for controlling multidrug resistant S. Pullorum, it is important to understand phage biology. A lytic Pullorum was isolated and characterized from chicken feces, its whole genome sequenced analyzed. new phage-vB_SPuM_SP116 (in brief SP116)- using SPu-116 host belongs Myoviridae A1 group. Phage SP116 had a effect on 27 37 (72.9%) different serotypes clinical Salmonella strains. It showed high bactericidal activity in killing all pathogens cultures containing 5 × 105 cfu/mL achieved more than 6.58 5.97 log unit reductions 5 × 106 cfu/mL 5 × 107 cfu/mL, respectively. The one-step growth curve that the burst size up 118 pfu/bacterial cell. Complete sequence analysis revealed linear, double-stranded DNA 87,510 bp with an average G + C content 38.84%, including 128 predicted open reading frames (ORFs) 22 tRNA genes. classified Felix O1 virus based upon general characterization genomic information. Regarding efficacy preventing especially infection lack any bacterial virulence, resistance, lysogenesis genes, could be potential alternative candidate treatment infections.