Scalable approach for the production of functional DNA based gold nanoprobes

摘要: Abstract Nanoparticle based systems, in particular gold nanoparticles (AuNPs), provide for simple colorimetric detection of molecular biomarkers, such as DNA, RNA. These systems rely on the functionalization AuNPs with ssDNA oligonucleotides requiring strenuous laboratory centrifugation steps not compatible industrial scale up. Here, we demonstrate potential dia-ultrafiltration purification Au-nanoprobes. We show that can be regarded better alternative to centrifugation, allowing a less intensive sample manipulation, easier transposable scale. The was performed by using membranes regenerated cellulose nominal weight cut-off (MWCO) 10 kDa and processing strategy which combined subsequent cleaning concentration steps. Instead permeation flux decline typically found ultrafiltration processes operated under modes, Au-nanoprobes followed subtle increase fluxes. This effect ascribed improved external mass transfer conditions near membrane surface, prompted decrease overall solute retentate over process time. allowed total retention AuNPS, yielding nanoprobes capable higher signal noise ratios. Proof-of-concept directed at synthesis identification members Mycobacterium tuberculosis complex cause humans.