Protocol for rye anther culture

摘要: Rye (Secale cereale L.) is a cross-pollinated cereal, which has strong self-incompatibility system. Population varieties of rye are highly heterozygous. Genes for self-fertility can, however, be introduced from wild relatives purposes hybrid breeding. In population breeding, selfing detrimental, as suffers severe inbreeding depression. The protocol anther culture was developed cross-pollinating breeding and genetic marker research. been generally considered to recalcitrant culture. source self-compatibility, weedy relative S. vavilovii (L), shown better response (Immonen, 1999). However, the use self-fertile species in would problematic therefore needed cultivated rye. motivation developing broadly applicable three-fold: production homozygous doubled haploids could facilitate process because, although time testing combining ability cannot reduced, differentiation quantitative traits more effective with DH lines; studies materials make development mapping populations faster easier than otherwise case obligatory cross-breeding crops; potential means fixing trait or increasing its prevalence population, providing that can selected either visually through marker-assisted selection at plantlet stage, circumvented. latter reached recurrent scheme provided method used subsequent cycles economical efficient, restrictions overcome.