作者: Xiangfu Zhong , Albert Pla , Simon Rayner
DOI: 10.1093/BIOINFORMATICS/BTZ806
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摘要: Motivation The existence of complex subpopulations miRNA isoforms, or isomiRs, is well established. While many tools exist for investigating isomiR populations, they differ in how characterize an isomiR, making it difficult to compare results across different tools. Thus, there a need more comprehensive and systematic standard defining isomiRs. Such would allow investigation population structure progressively refined sub-populations, permitting the identification subtle changes between conditions leading improved understanding processes that generate these differences. Results We developed Jasmine, software tool incorporates hierarchal framework characterizing populations. Jasmine Java application can process raw read data fastq/fasta format, mapped reads SAM format produce detailed characterization reveal not apparent miRNA-Seq analysis pipeline. Availability implemented R freely available at bitbucket https://bitbucket.org/bipous/jasmine/src/master/. Supplementary information are Bioinformatics online.