Relationships, among the steroids, of anti-inflammatory properties and inhibition of prostaglandin production and arachidonic acid release by transformed mouse fibroblasts.

摘要: Anti-inflammatory steroids inhibit the serum-stimulated prostaglandin synthesis and release of [3H]arachidonic acid by methylcholanthrene-transformed mouse fibroblast, MC5-5. The half-maximal concentration for inhibition both effects parallels relative anti-inflammatory potencies these steroids. fatty cyclooxygenase activities microsomal fractions isolated from cells that had been pretreated with dexamethasone were similar to untreated cells. Intracellular as well extracellular levels prostaglandins in inhibited decreased. seem be blocking deacylation phospholipids or transport arachidonic acid, after cyclooxygenase.