Electrochemical investigations of the interaction of metal chelates with DNA. 3. Electrogenerated chemiluminescent investigation of the interaction of tris(1,10-phenanthroline)ruthenium(II) with DNA.

摘要: The electrogenerated chemiluminescence (ECL) that results from the oxidation of tris(1,10-phenanthroline)ruthenium(II), at a gold electrode in presence oxalate, was used to investigate interaction Ru(II) chelate with calf thymus DNA. decrease ECL emission excited state, Ru(phen)3(2+*), DNA, is ascribed binding DNA strand. An titration metal complex allowed determination equilibrium constant (K) and binding-site size (s) for association Ru(phen)3(2+), under assumption only free contributes observed emission. In 25 mM Na2C2O4, 2 phosphate buffer, pH 5, 0.05% Tween-20, Triton X-100, regression based on McGhee/von Hippel model, which accounts base pair gaps between sites, yielded K = 8.1 (+/- 0.2) x 10(3) M-1 s 4 bp.