Lymphokine-activated killer (LAK) cells. II. Delineation of distinct murine LAK-precursor subpopulations.

摘要: Lymphokine-activated killer (LAK) cells can lyse a number of tumor target regardless whether the tumors are natural (NK) sensitive or resistant. LAK also autologous lymphoblasts that have been modified with 2,4,6-trinitrobenzene sulfonic acid (TNBS). In this study, we examined surface markers murine precursors. It was found depletion Thy 1- Lyt 2-bearing precursor abolished ability spleen to generate against TNBS-self, but had no effect on generation cells. Depletion asialo-GM1 (AGM1)-bearing precursors all tested. Normal were fractionated Percoll density gradient and two fractions examined: fraction (Fxn) 3, which is enriched for NK activity depleted cytotoxic T lymphocytes (CTL), Fxn 5, CTL. Both capable generating LAK, although 5 required relatively larger amount interleukin 2 (IL 2). Upon examination in these it 3 giving rise Thy-1-, Lyt-2-, AGM1+, whereas Thy-1+, Lyt-2+, AGM1+. The TNBS-self AGM1+ both fractions. time kinetics different, showing much earlier kinetics. These data delineate at least different defined by their buoyant density, markers, susceptible may resolve confusion literature regarding phenotype