Enhancement of the Immunoadjuvant Activity of Immunostimulatory DNA Sequence By Liposomal Delivery

摘要: Bacterial DNA and its synthetic immunostimulatory oligodeoxynucleotides’ analogs (ISS-ODN or CpG motifs) are potent stimulators of both innate immunity specific adaptive immune responses (1–5). ISSODNs directly activate monocytes/macrophages, dendritic cells, natural killer (NK) cells B (6–10), induce the production proinflammatory cytokines (e.g., IL-6, IL-12, IFNs, TNFa) (4,6,7,11,12), upregulate expression MHC I, II co-stimulatory molecules (9,13). In animal studies, ISS-ODNs exhibit strong Th1 (2,14–18) mucosal adjuvanticity to a wide range antigens (2,3,14,19–26) allergens (27–30). Furthermore, pretreatment with ISS-ODN, even without concomitant administration relevant antigens, can afford protection (for about 2 wk) against subsequent infection intracellular pathogens (31–35), indicating activation immunity.