作者: Iyoko Katoh , Anna Mírová , Shun-ichi Kurata , Yasushi Murakami , Kenji Horikawa
DOI: 10.1593/NEO.11794
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摘要: The human and Old World primate genomes possess conserved endogenous retrovirus sequences that have been implicated in evolution, reproduction, carcinogenesis. Human (HERV)-K with 5′LTR-gag-pro-pol-env-rec/np9-3′LTR represents the newest family integrated into genome 1 to 5 million years ago. Although a high-level expression of HERV-K melanomas, breast cancers, teratocarcinomas has demonstrated, mechanism lineage-specific activation long terminal repeat (LTR) remains obscure. We studied chromosomal MeWo melanoma cells comparison basal embryonic kidney 293 (HEK293) cells. Cloned LTR (HML-2.HOM) was also characterized by mutation transactivation experiments. detected multiple transcriptional initiator (Inr) sites rapid amplification complementary DNA ends (5′ RACE). HEK293 showed different Inr usage. most potent associated TATA box three binding motifs microphthalmia-associated transcription factor (MITF). Both cloned function were strongly activated transfection MITF-M, melanocyte/melanoma-specific isoform MITF. Coexpression MITF core antigen retinal pigmented epithelium an immunofluorescence analysis. malignant lines MeWo, G361, SK-MEL-28 enhanced compared normal melanocytes, level MITF-M messenger RNA persisted from transformed melanocytes. Thus, may be prerequisite for cell LTR, leading melanomas.