Activation of the Long Terminal Repeat of Human Endogenous Retrovirus K by Melanoma-Specific Transcription Factor MITF-M

作者: Iyoko Katoh , Anna Mírová , Shun-ichi Kurata , Yasushi Murakami , Kenji Horikawa

DOI: 10.1593/NEO.11794

关键词:

摘要: The human and Old World primate genomes possess conserved endogenous retrovirus sequences that have been implicated in evolution, reproduction, carcinogenesis. Human (HERV)-K with 5′LTR-gag-pro-pol-env-rec/np9-3′LTR represents the newest family integrated into genome 1 to 5 million years ago. Although a high-level expression of HERV-K melanomas, breast cancers, teratocarcinomas has demonstrated, mechanism lineage-specific activation long terminal repeat (LTR) remains obscure. We studied chromosomal MeWo melanoma cells comparison basal embryonic kidney 293 (HEK293) cells. Cloned LTR (HML-2.HOM) was also characterized by mutation transactivation experiments. detected multiple transcriptional initiator (Inr) sites rapid amplification complementary DNA ends (5′ RACE). HEK293 showed different Inr usage. most potent associated TATA box three binding motifs microphthalmia-associated transcription factor (MITF). Both cloned function were strongly activated transfection MITF-M, melanocyte/melanoma-specific isoform MITF. Coexpression MITF core antigen retinal pigmented epithelium an immunofluorescence analysis. malignant lines MeWo, G361, SK-MEL-28 enhanced compared normal melanocytes, level MITF-M messenger RNA persisted from transformed melanocytes. Thus, may be prerequisite for cell LTR, leading melanomas.

参考文章(52)
Maya Golan, Amnon Hizi, James H. Resau, Neora Yaal-Hahoshen, Hadar Reichman, Iafa Keydar, Ilan Tsarfaty, Human endogenous retrovirus (HERV-K) reverse transcriptase as a breast cancer prognostic marker. Neoplasia. ,vol. 10, pp. 521- 533 ,(2008) , 10.1593/NEO.07986
Amanda Gonçalves dos Santos Silva, Herbert Alexander Graves, Amanda Guffei, Tatiana Iervolino Ricca, Renato Arruda Mortara, Miriam Galvonas Jasiulionis, Sabine Mai, Telomere-Centromere-Driven Genomic Instability Contributes to Karyotype Evolution in a Mouse Model of Melanoma Neoplasia. ,vol. 12, pp. 11- 19 ,(2010) , 10.1593/NEO.91004
Timothy J. Hemesath, E. Roydon Price, Clifford Takemoto, Tina Badalian, David E. Fisher, MAP kinase links the transcription factor Microphthalmia to c-Kit signalling in melanocytes Nature. ,vol. 391, pp. 298- 301 ,(1998) , 10.1038/34681
Oliver Schanab, Johannes Humer, Andreas Gleiss, Mario Mikula, Sanda Sturlan, Susanne Grunt, Ichiro Okamoto, Thomas Muster, Hubert Pehamberger, Andrea Waltenberger, None, Expression of human endogenous retrovirus K is stimulated by ultraviolet radiation in melanoma. Pigment Cell & Melanoma Research. ,vol. 24, pp. 656- 665 ,(2011) , 10.1111/J.1755-148X.2011.00860.X
Jens Mayer, Marlies Sauter, Alexander Rácz, Daniela Scherer, Nikolaus Mueller-Lantzsch, Eckart Meese, An almost-intact human endogenous retrovirus K on human chromosome 7. Nature Genetics. ,vol. 21, pp. 257- 258 ,(1999) , 10.1038/6766
Menashe Bar-Eli, Role of AP-2 in tumor growth and metastasis of human melanoma. Cancer and Metastasis Reviews. ,vol. 18, pp. 377- 385 ,(1999) , 10.1023/A:1006377309524
Yann Cheli, Mickael Ohanna, Robert Ballotti, Corine Bertolotto, Fifteen‐year quest for microphthalmia‐associated transcription factor target genes Pigment Cell & Melanoma Research. ,vol. 23, pp. 27- 40 ,(2010) , 10.1111/J.1755-148X.2009.00653.X
Nahoko Fukunishi, Iyoko Katoh, Yoshiya Tomimori, Keiichi Tsukinoki, Ryu-Ichiro Hata, Atsuhito Nakao, Yoji Ikawa, Shun-ichi Kurata, Induction of ΔNp63 by the Newly Identified Keratinocyte-Specific Transforming Growth Factor β Signaling Pathway with Smad2 and IκB Kinase α in Squamous Cell Carcinoma Neoplasia. ,vol. 12, pp. 969- 979 ,(2010) , 10.1593/NEO.101054
C Patience, G R Simpson, A A Colletta, H M Welch, R A Weiss, M T Boyd, Human endogenous retrovirus expression and reverse transcriptase activity in the T47D mammary carcinoma cell line Journal of Virology. ,vol. 70, pp. 2654- 2657 ,(1996) , 10.1128/JVI.70.4.2654-2657.1996